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为探究低温等离子体对羊肚菌栽培基质的灭菌效果，以羊肚菌栽培基质为研究对象，采用高通量测序技术，比较分析了低温储存、室温储存及低温等离子体处理后基质中细菌群落组成与多样性变化。结果显示，低温等离子体处理后细菌群落物种多样性、丰富度和均匀度均显著降低，最高下降幅度分别为32%,34%及9%。科水平上，处理后伯克氏菌科相对丰度升高。属水平上，处理后优势菌属为水杆菌属。等离子体对链霉菌属Streptomyces、黄单胞菌属Xanthomonas、苍白杆菌属Ochrobactrum、帕拉伯克霍尔德氏菌Paraburkholderia等多种致病菌的杀菌率均在99%以上。首次将常压低温等离子体技术应用于羊肚菌基质灭菌，可有效杀灭多种致病菌，在羊肚菌栽培基质的灭菌和保鲜方面具有良好的应用前景。
Abstract：Cultivation substrate of Agaricus Morchella was used to investigate the sterilization effect of bacteria through the low-temperature plasma technology carried out at atmospheric pressure.High-throughput sequencing technology was performed to analyze the changes of bacterial community, such as composition and diversity in the substrate, before and after treatments of low-temperature and room-temperature as well as low-temperature plasma. Our results showed that the diversity, richness and evenness of bacterial community were significantly reduced after treatment by low-temperature plasma, with the largest decrement rate up to 32%, 34% and 9%,respectively. Among different families detected from the bacterial community, however, relative richness of Burkholderiaceae increased continuously after treatment while among the genera, the dominant bacterium was Aquabacterium after treatment. The bactericidal rate of low-temperature plasma treatment against a variety of pathogenic bacteria such as Streptomyces, Xanthomonas,Ochrobactrum, and Paraburkholderia was above 99%. This study, as the first time, applied the new technology by using the atmospheric low temperature plasma for substrate sterilization of Morchella,which could effectively kill a variety of pathogenic bacteria, thereafter, demonstrating a good example and prospects for the future applications of above technology to the effective sterilization and preservation of Morchella cultivation substrates.
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基本信息:
DOI：10.13341/j.jfr.2023.1660
中图分类号:S646.7
引用信息:
[1]贺雪峰,樊灏,周桂灵等.常压低温等离子体对羊肚菌栽培基质细菌群落的影响[J].菌物研究,2025,23(03):223-230.DOI:10.13341/j.jfr.2023.1660.
基金信息:
西安市科学技术局农业技术研发项目（21NYYF0041）