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鸡枞菌菌丝体在培养基上不能扭结发育成子实体。为揭示子实体发育的潜在关联物质，应用液相质谱（LC-MS）技术，对皱皮粗柄鸡枞菌Termitomyces robustus子实体及其纯培养菌丝体的代谢物谱进行了分析。在子实体及菌丝体中分别检测到1 424和1 457个物质，其中子实体特有物质有212个，菌丝体特有物质245个；二者共有显著差异物质543个，包括上调物质377个，下调物质166个。利用Cytoscape软件展示了子实体及菌丝体特有物质间中，显著相关的58个共有差异物质间的网络关联。KEGG富集分析表明，23个特有物质（菌丝体14个，子实体9个）及38个差异物质（上调27个，下调11个）分别通过4条及9条共有通路发生了上下游关联。综上，菌丝体与子实体特有物质间、上调与下调物质间分别存在网络关联，推测这些物质特别是子实体特有及上调物质对子实体的发育具有一定的调控作用；其中126个物质（相对含量≥2的65个，富集到通路的61个）在菌丝体及子实体中显著或紧密相关，推测这些物质对子实体的发育有重要的调控作用。以上结果为今后进一步分析鸡枞菌子实体发育相关的信号分子，筛选子实体发育的促生物质，为规模化人工培养鸡枞菌、开发抗肿瘤等相关药物等研究提供了理论依据。
Abstract：Termitomyces mycelia could not twist to develop into a fruiting body on the culture media.In order to reveal those substances potentially relating to development of fruiting bodies, metabolites isolated from the fruiting bodies and purely cultured mycelia of Termitomyces robustus were analyzed by using liquid chromatography-mass spectrometry(LC-MS). A total of 1 424 and 1 457 substances were detected within the above two tissues of fungi respectively, of which 212 were specific to fruiting bodies while 245 were specific to mycelia. There existed 543 kinds of significantly differential substances between the fruiting body and the mycelium, including 377 up-regulated and 166 down-regulated substances. Cytoscape software was used to demonstrate the network associations between the above tissue-specific substances that revealed a presence of 58 commonly differential substances. KEGG enrichment analysis indicated an occurrence of upstream and downstream regulation association for 23 tissue-specific substances(14 from mycelia and nine from fruiting bodies) and 38 differential substances(27 up-regulated and 11 down-regulated) respectively under four and nine common pathways. The above findings, thereafter, enable us to suggest that these substances, especially fruitingbody-specific substances and up-regulated substances, have certain regulatory effects on the development of fruiting body, in which 126 substances(65 showing the relative content ≥2 and 61 enriched under the pathways) revealed significant or close relationships between mycelia and fruiting bodies. Our results thus provide a theoretical basis for further analysis of signal molecules and screening of facilitatory metabolites in relation to the development of Termitomyces fruiting bodies, large-scale artificial cultures of Termitomyces and drug development of the anti-tumor and others as well.
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基本信息:
DOI：10.13341/j.jfr.2024.1797
中图分类号:S646
引用信息:
[1]刘夏,王成凤,张曦予等.皱皮粗柄鸡枞菌子实体发育的关联及调控物质分析[J].菌物研究,2025,23(02):162-174.DOI:10.13341/j.jfr.2024.1797.
基金信息:
国家自然科学基金项目（31560575）; 云南省自然科学基金项目（2014FA020）